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empty vector control  (OriGene)


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    Structured Review

    OriGene empty vector control
    Empty Vector Control, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+vector/Parp11+(NM_181402)+Mouse+Tagged+ORF+Clone/bio_rxiv__64898__2026__06__01__729331-208-6-12
    Average 94 stars, based on 1 article reviews
    empty vector control - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: A CPEB2/ANGPTL3 feedback loop promotes the progression of podocyte injury in nephrotic syndrome.
    Article Snippet: Transient transfections were performed with Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. The Cpeb2 mouse expression vector (Cat. No. MR225994L4) and the control vector (Cat. No. PS100093) were purchased from OriGene (Rockville, MD, USA). .. Cpeb2 Mouse siRNA Oligo Duplex (Cat. No. SR416598), Angptl3 Mouse siRNA Oligo Duplex (Cat. No. SR414829) and Eif3d Mouse siRNA Oligo Duplex (Cat. No. SR417145) were purchased from OriGene (Rockville, MD, USA).

    Article Title: RING box protein-1 promotes the metastasis of cervical cancer through regulating matrix metalloproteinases via PI3K/AKT signaling pathway.
    Article Snippet: the estimated age-related incidence of cervical cancer was 13.1 per 100,000 women, with significant regional variations (Akinyemiju 2012).. CC is commonly referred to as a “disease of disparity” because of the stark differences in incidence and mortality between high-income countries (HIC) and lowand middle-income countries (LMIC).. Undoubtedly, LIMCs bear the weight of this statistic, accounting for about 85% of all new CC cases and 90% of CC-related deaths worldwide (Vu et al. 2018).

    Control:

    Article Title: A CPEB2/ANGPTL3 feedback loop promotes the progression of podocyte injury in nephrotic syndrome.
    Article Snippet: Transient transfections were performed with Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. The Cpeb2 mouse expression vector (Cat. No. MR225994L4) and the control vector (Cat. No. PS100093) were purchased from OriGene (Rockville, MD, USA). .. Cpeb2 Mouse siRNA Oligo Duplex (Cat. No. SR416598), Angptl3 Mouse siRNA Oligo Duplex (Cat. No. SR414829) and Eif3d Mouse siRNA Oligo Duplex (Cat. No. SR417145) were purchased from OriGene (Rockville, MD, USA).

    Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
    Article Snippet: .. FOSL1 human GFP-tagged ORF clone (cat. no. RG202104) and control vector (cat. no. PS100010), as well as FOSL1-Myc-DDK (FLAG)-tagged ORF (cat. no. RC202104) and control vector (cat. no. PS100001) were purchased from OriGene. .. Human pLVX puro TRIM21-GFP (cat. no.116941), pmCherry-C1-mTrim21(cat. no.105516), pmCherry-C1mTrim21∆RING-Box (cat. no. 105517), pmCherry-C1mTrim21-∆PRYSPRY (cat. no. 105518) were obtained from Addgene.

    Article Title: Caffeine improves hypoxia/reoxygenation induced neuronal cell injury through inhibiting cellular ferroptosis: an in vitro study.
    Article Snippet: Objective: Ferroptosis, a regulated cell death pathway driven by lipid peroxidation and iron overload, is implicated in neuronal injury caused by hypoxia/reoxygenation (H/R).. Caffeine, a widely consumed psychoactive compound, has shown neuroprotective effects in various central nervous system disorders, but its role in regulating ferroptosis remains unclear.. This study investigates the neuroprotective effects of caffeine on ferroptosis and its regulation of ACSL4, a key ferroptosis-related protein.

    Article Title: Feedforward miR-181d degradation modulates population variance of methyl-guanine methyl transferase and temozolomide resistance.
    Article Snippet: .. The corresponding negative control cell lines were generated by transfecting an empty control vector (OriGene) into CMK3 or BT-83 cells and selected with neomycin. .. PNPT1 CRISPR lentivirus was generated by the transfection of pLenti-CRISPR v2-sgPNPT1-2 lentiviral vector into HEK293T cells with psPAX2 and pCMV-VSV-G (Addgene) at a ratio of 10:9:1.

    Article Title: Feedforward miR-181d degradation modulates population variance of methyl-guanine methyl transferase and temozolomide resistance
    Article Snippet: .. The corresponding negative control cell lines were generated by transfecting an empty control vector (OriGene) into CMK3 or BT-83 cells and selected with neomycin. .. PNPT1 CRISPR lentivirus was generated by the transfection of pLenti-CRISPR v2-sgPNPT1-2 lentiviral vector into HEK293T cells with psPAX2 and pCMV-VSV-G (Addgene) at a ratio of 10:9:1.

    Article Title: RING box protein-1 promotes the metastasis of cervical cancer through regulating matrix metalloproteinases via PI3K/AKT signaling pathway.
    Article Snippet: the estimated age-related incidence of cervical cancer was 13.1 per 100,000 women, with significant regional variations (Akinyemiju 2012).. CC is commonly referred to as a “disease of disparity” because of the stark differences in incidence and mortality between high-income countries (HIC) and lowand middle-income countries (LMIC).. Undoubtedly, LIMCs bear the weight of this statistic, accounting for about 85% of all new CC cases and 90% of CC-related deaths worldwide (Vu et al. 2018).

    Article Title: Makorin Ring Finger Protein 1 Inhibits Cell Proliferation in Renal Angiomyolipoma via the ERK/MAPK Signaling Pathway
    Article Snippet: .. Cells were then transfected with either a MKRN1-overexpressing plasmid or a control vector (OriGene, Rockville, MD, USA) using LipofectamineTM 2000 Reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s protocol. ..

    Plasmid Preparation:

    Article Title: A CPEB2/ANGPTL3 feedback loop promotes the progression of podocyte injury in nephrotic syndrome.
    Article Snippet: Transient transfections were performed with Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. The Cpeb2 mouse expression vector (Cat. No. MR225994L4) and the control vector (Cat. No. PS100093) were purchased from OriGene (Rockville, MD, USA). .. Cpeb2 Mouse siRNA Oligo Duplex (Cat. No. SR416598), Angptl3 Mouse siRNA Oligo Duplex (Cat. No. SR414829) and Eif3d Mouse siRNA Oligo Duplex (Cat. No. SR417145) were purchased from OriGene (Rockville, MD, USA).

    Article Title: Caffeine improves hypoxia/reoxygenation induced neuronal cell injury through inhibiting cellular ferroptosis: an in vitro study.
    Article Snippet: Objective: Ferroptosis, a regulated cell death pathway driven by lipid peroxidation and iron overload, is implicated in neuronal injury caused by hypoxia/reoxygenation (H/R).. Caffeine, a widely consumed psychoactive compound, has shown neuroprotective effects in various central nervous system disorders, but its role in regulating ferroptosis remains unclear.. This study investigates the neuroprotective effects of caffeine on ferroptosis and its regulation of ACSL4, a key ferroptosis-related protein.

    Article Title: Feedforward miR-181d degradation modulates population variance of methyl-guanine methyl transferase and temozolomide resistance.
    Article Snippet: .. The corresponding negative control cell lines were generated by transfecting an empty control vector (OriGene) into CMK3 or BT-83 cells and selected with neomycin. .. PNPT1 CRISPR lentivirus was generated by the transfection of pLenti-CRISPR v2-sgPNPT1-2 lentiviral vector into HEK293T cells with psPAX2 and pCMV-VSV-G (Addgene) at a ratio of 10:9:1.

    Article Title: Feedforward miR-181d degradation modulates population variance of methyl-guanine methyl transferase and temozolomide resistance
    Article Snippet: .. The corresponding negative control cell lines were generated by transfecting an empty control vector (OriGene) into CMK3 or BT-83 cells and selected with neomycin. .. PNPT1 CRISPR lentivirus was generated by the transfection of pLenti-CRISPR v2-sgPNPT1-2 lentiviral vector into HEK293T cells with psPAX2 and pCMV-VSV-G (Addgene) at a ratio of 10:9:1.

    Article Title: RING box protein-1 promotes the metastasis of cervical cancer through regulating matrix metalloproteinases via PI3K/AKT signaling pathway.
    Article Snippet: the estimated age-related incidence of cervical cancer was 13.1 per 100,000 women, with significant regional variations (Akinyemiju 2012).. CC is commonly referred to as a “disease of disparity” because of the stark differences in incidence and mortality between high-income countries (HIC) and lowand middle-income countries (LMIC).. Undoubtedly, LIMCs bear the weight of this statistic, accounting for about 85% of all new CC cases and 90% of CC-related deaths worldwide (Vu et al. 2018).

    Article Title: Makorin Ring Finger Protein 1 Inhibits Cell Proliferation in Renal Angiomyolipoma via the ERK/MAPK Signaling Pathway
    Article Snippet: .. Cells were then transfected with either a MKRN1-overexpressing plasmid or a control vector (OriGene, Rockville, MD, USA) using LipofectamineTM 2000 Reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s protocol. ..

    Negative Control:

    Article Title: Feedforward miR-181d degradation modulates population variance of methyl-guanine methyl transferase and temozolomide resistance.
    Article Snippet: .. The corresponding negative control cell lines were generated by transfecting an empty control vector (OriGene) into CMK3 or BT-83 cells and selected with neomycin. .. PNPT1 CRISPR lentivirus was generated by the transfection of pLenti-CRISPR v2-sgPNPT1-2 lentiviral vector into HEK293T cells with psPAX2 and pCMV-VSV-G (Addgene) at a ratio of 10:9:1.

    Article Title: Feedforward miR-181d degradation modulates population variance of methyl-guanine methyl transferase and temozolomide resistance
    Article Snippet: .. The corresponding negative control cell lines were generated by transfecting an empty control vector (OriGene) into CMK3 or BT-83 cells and selected with neomycin. .. PNPT1 CRISPR lentivirus was generated by the transfection of pLenti-CRISPR v2-sgPNPT1-2 lentiviral vector into HEK293T cells with psPAX2 and pCMV-VSV-G (Addgene) at a ratio of 10:9:1.

    Generated:

    Article Title: Feedforward miR-181d degradation modulates population variance of methyl-guanine methyl transferase and temozolomide resistance.
    Article Snippet: .. The corresponding negative control cell lines were generated by transfecting an empty control vector (OriGene) into CMK3 or BT-83 cells and selected with neomycin. .. PNPT1 CRISPR lentivirus was generated by the transfection of pLenti-CRISPR v2-sgPNPT1-2 lentiviral vector into HEK293T cells with psPAX2 and pCMV-VSV-G (Addgene) at a ratio of 10:9:1.

    Article Title: Feedforward miR-181d degradation modulates population variance of methyl-guanine methyl transferase and temozolomide resistance
    Article Snippet: .. The corresponding negative control cell lines were generated by transfecting an empty control vector (OriGene) into CMK3 or BT-83 cells and selected with neomycin. .. PNPT1 CRISPR lentivirus was generated by the transfection of pLenti-CRISPR v2-sgPNPT1-2 lentiviral vector into HEK293T cells with psPAX2 and pCMV-VSV-G (Addgene) at a ratio of 10:9:1.

    Transfection:

    Article Title: Makorin Ring Finger Protein 1 Inhibits Cell Proliferation in Renal Angiomyolipoma via the ERK/MAPK Signaling Pathway
    Article Snippet: .. Cells were then transfected with either a MKRN1-overexpressing plasmid or a control vector (OriGene, Rockville, MD, USA) using LipofectamineTM 2000 Reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s protocol. ..



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    OriGene scramble control shrna
    <t>TCF7</t> regulates pro−caspase−8 expression in T lymphocytes and is significantly reduced in COPD. ( A ) Immunofluorescence co−staining of control human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Scale bar is 50 μm. ( B ) Immunofluorescence co−staining of COPD human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Note the marked reduction in both TCF7 and caspase−8 signals compared to the control. Scale bar is 50 μm. ( C ) Representative Western blot images of TCF7 (50 kDa), pro−caspase−8 (55 kDa), and internal control β−tubulin (55 kDa) in wild type (WT) and TCF7 knockout (KO) Jurkat T cells. ( D ) Quantitative densitometric analysis of TCF7 protein levels comparing WT and KO groups. ( E ) Quantitative densitometric analysis of pro−caspase−8 protein levels comparing WT and KO groups. ( F ) Representative Western blot images of TCF7 and β−tubulin in primary T lymphocytes isolated from the peripheral blood of healthy donors (Control) and patients with COPD (Model). ( G ) Quantitative densitometric analysis of TCF7 protein levels in human primary T lymphocytes. ( H ) Representative Western blot images of TCF7 and β−tubulin protein levels in Jurkat T cells across four experimental conditions including Control, <t>shRNA,</t> shRNA plus TCF7 Rescue construct, and shRNA plus Empty Vector. ( I ) Quantitative densitometric analysis of TCF7 protein levels across the four experimental rescue groups. ( J ) Representative Western blot images of pro−caspase−8 and β−tubulin protein levels across the same four experimental conditions in Jurkat T cells. ( K ) Quantitative densitometric analysis of pro−caspase−8 protein levels across the four experimental rescue groups. Data in the bar charts are presented as mean ± SD ( n = 4 for primary human cells, n = 3 for cell line experiments). Statistical significance was assessed using Student’s t test with Welch’s correction where appropriate (* p < 0.05, *** p < 0.001, ns indicates not significant).
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    Genechem non targeting control vectors shnc
    <t>TCF7</t> regulates pro−caspase−8 expression in T lymphocytes and is significantly reduced in COPD. ( A ) Immunofluorescence co−staining of control human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Scale bar is 50 μm. ( B ) Immunofluorescence co−staining of COPD human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Note the marked reduction in both TCF7 and caspase−8 signals compared to the control. Scale bar is 50 μm. ( C ) Representative Western blot images of TCF7 (50 kDa), pro−caspase−8 (55 kDa), and internal control β−tubulin (55 kDa) in wild type (WT) and TCF7 knockout (KO) Jurkat T cells. ( D ) Quantitative densitometric analysis of TCF7 protein levels comparing WT and KO groups. ( E ) Quantitative densitometric analysis of pro−caspase−8 protein levels comparing WT and KO groups. ( F ) Representative Western blot images of TCF7 and β−tubulin in primary T lymphocytes isolated from the peripheral blood of healthy donors (Control) and patients with COPD (Model). ( G ) Quantitative densitometric analysis of TCF7 protein levels in human primary T lymphocytes. ( H ) Representative Western blot images of TCF7 and β−tubulin protein levels in Jurkat T cells across four experimental conditions including Control, <t>shRNA,</t> shRNA plus TCF7 Rescue construct, and shRNA plus Empty Vector. ( I ) Quantitative densitometric analysis of TCF7 protein levels across the four experimental rescue groups. ( J ) Representative Western blot images of pro−caspase−8 and β−tubulin protein levels across the same four experimental conditions in Jurkat T cells. ( K ) Quantitative densitometric analysis of pro−caspase−8 protein levels across the four experimental rescue groups. Data in the bar charts are presented as mean ± SD ( n = 4 for primary human cells, n = 3 for cell line experiments). Statistical significance was assessed using Student’s t test with Welch’s correction where appropriate (* p < 0.05, *** p < 0.001, ns indicates not significant).
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    Image Search Results


    TCF7 regulates pro−caspase−8 expression in T lymphocytes and is significantly reduced in COPD. ( A ) Immunofluorescence co−staining of control human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Scale bar is 50 μm. ( B ) Immunofluorescence co−staining of COPD human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Note the marked reduction in both TCF7 and caspase−8 signals compared to the control. Scale bar is 50 μm. ( C ) Representative Western blot images of TCF7 (50 kDa), pro−caspase−8 (55 kDa), and internal control β−tubulin (55 kDa) in wild type (WT) and TCF7 knockout (KO) Jurkat T cells. ( D ) Quantitative densitometric analysis of TCF7 protein levels comparing WT and KO groups. ( E ) Quantitative densitometric analysis of pro−caspase−8 protein levels comparing WT and KO groups. ( F ) Representative Western blot images of TCF7 and β−tubulin in primary T lymphocytes isolated from the peripheral blood of healthy donors (Control) and patients with COPD (Model). ( G ) Quantitative densitometric analysis of TCF7 protein levels in human primary T lymphocytes. ( H ) Representative Western blot images of TCF7 and β−tubulin protein levels in Jurkat T cells across four experimental conditions including Control, shRNA, shRNA plus TCF7 Rescue construct, and shRNA plus Empty Vector. ( I ) Quantitative densitometric analysis of TCF7 protein levels across the four experimental rescue groups. ( J ) Representative Western blot images of pro−caspase−8 and β−tubulin protein levels across the same four experimental conditions in Jurkat T cells. ( K ) Quantitative densitometric analysis of pro−caspase−8 protein levels across the four experimental rescue groups. Data in the bar charts are presented as mean ± SD ( n = 4 for primary human cells, n = 3 for cell line experiments). Statistical significance was assessed using Student’s t test with Welch’s correction where appropriate (* p < 0.05, *** p < 0.001, ns indicates not significant).

    Journal: International Journal of Molecular Sciences

    Article Title: Unfolding Immune Dysregulation in COPD: Identification of a Three-Gene Signature and Functional Validation of TCF7 in Human Lung Tissue and T Lymphocytes

    doi: 10.3390/ijms27104231

    Figure Lengend Snippet: TCF7 regulates pro−caspase−8 expression in T lymphocytes and is significantly reduced in COPD. ( A ) Immunofluorescence co−staining of control human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Scale bar is 50 μm. ( B ) Immunofluorescence co−staining of COPD human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Note the marked reduction in both TCF7 and caspase−8 signals compared to the control. Scale bar is 50 μm. ( C ) Representative Western blot images of TCF7 (50 kDa), pro−caspase−8 (55 kDa), and internal control β−tubulin (55 kDa) in wild type (WT) and TCF7 knockout (KO) Jurkat T cells. ( D ) Quantitative densitometric analysis of TCF7 protein levels comparing WT and KO groups. ( E ) Quantitative densitometric analysis of pro−caspase−8 protein levels comparing WT and KO groups. ( F ) Representative Western blot images of TCF7 and β−tubulin in primary T lymphocytes isolated from the peripheral blood of healthy donors (Control) and patients with COPD (Model). ( G ) Quantitative densitometric analysis of TCF7 protein levels in human primary T lymphocytes. ( H ) Representative Western blot images of TCF7 and β−tubulin protein levels in Jurkat T cells across four experimental conditions including Control, shRNA, shRNA plus TCF7 Rescue construct, and shRNA plus Empty Vector. ( I ) Quantitative densitometric analysis of TCF7 protein levels across the four experimental rescue groups. ( J ) Representative Western blot images of pro−caspase−8 and β−tubulin protein levels across the same four experimental conditions in Jurkat T cells. ( K ) Quantitative densitometric analysis of pro−caspase−8 protein levels across the four experimental rescue groups. Data in the bar charts are presented as mean ± SD ( n = 4 for primary human cells, n = 3 for cell line experiments). Statistical significance was assessed using Student’s t test with Welch’s correction where appropriate (* p < 0.05, *** p < 0.001, ns indicates not significant).

    Article Snippet: Short hairpin RNA targeting human TCF7 (shRNA) and a scramble control shRNA were purchased from OriGene with Cat.No.TR30004.

    Techniques: Expressing, Immunofluorescence, Staining, Control, Western Blot, Knock-Out, Isolation, shRNA, Construct, Plasmid Preparation